Comparison of THP-1 Macrophages Viability in Different Types of Culture Vessel

The ox-LDL generated apoptotic bodies using THP-1 macrophage is a useful tool to study foam cell formation in atherosclerosis. However, the common problem cells negative control (i.e., absence of ox-LDL) undergo apoptosis. Therefore, type culture vessel was proposed be key factor contributing were differentiated into M1 macrophages 10 ng/μL PMA, 5 LPS, and 20 IFN-? while IL-4 IL-13 used differentiate M2 macrophages. Two types vessels (6-well plate T25 flask) subsequently stained Annexin V-FITC Propidium Iodide prior flow cytometry analysis. Interestingly, both cultured flask resulted significantly higher percentage viability compared 6-well [M1: 84.15% ± 4.39 vs 8.02% 1.55, p < 0.0001; M2: 95.95% 1.74 10.50% 0.05, 0.0001]. In summary, vital determining attributed surface area seeding density different vessels.